IL-12A is subunit of the cytokine IL-12 which acts on T- and natural killer cells, and has a broad array of biological activities.
IL-12 is a disulfide-linked heterodimer composed of the 35-kD subunit and a 40-kD subunit that is a member of the cytokine receptor family (1).
IL-12 is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells (1,2).
The responses of lymphocytes to IL-12 are mediated by the activator of transcription protein STAT4 (2).
Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity (3).
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlDetermined by the researcher.
Positive Control:HeLa Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human IL-12A.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to 18aa near the C-terminal of human IL-12A.
