The esophageal cancer-related gene 4 (ECRG4), also known as Augurin, was identified through differential display screening between esophageal cancer and normal tissue.
Hypermethylation of CpG sites in the ECRG4 promoter correlate with ECRG4 downregulation in numerous esophageal squamous cell carcinoma tissues.
In transfected cells, overexpression of ECRG4 induced G1 arrest, dephosphorylation of Rb and decreased expression of cyclins D1 and D3.
Increased expression of ECRG4 in aged mouse brain is observed compared with young mouse brain.
The increased ECRG4 level was accompanied by elevated senescence-associated acidic beta-galactosidase activity, suggesting that ECRG4 is involved in cellular differentiation and senescence.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunocytochemistry: 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:HeLa Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human ECRG4. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 20aa from human ECRG4 at C-terminal.
