The E2F transcription factor 3 (E2F3) is a member of a small family of transcription factors that function through binding of DP interaction partner proteins.
E2F3 recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle (1).
Like the related E2F1 and E2F2, E2F3 is essential for cellular proliferation and progression through the cell cycle (2).
Altered copy number and activity of this gene have been observed in a number of human cancers (3).
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 20ug/mlWestern Blot: 1-2ug/mlImmunohistochemistry (paraffin): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human Lymph Node Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human E2F3 Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide corresponding to 15aa near the center of human E2F3.
