DELE is a recently identified DAP3-binding protein that is thought to be important in the induction of death receptor-mediated apoptosis.
Transfected cells that stably express DELE were found to be susceptible to apoptosis induction by TNF-a and TRAIL, whereas reducing DELE expression by siRNA rescued these cells from apoptosis induction.
Furthermore, the reduction of DELE expression also inhibited the activation of caspase-3, caspase-8 and caspase-9 following stimulation by TNF-a, anti-Fas, or TRAIL, indicating the importance of DELE in apoptosis mediated by death receptors.
Applications:Suitable for use in Immunofluorescence, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 20ug/mlWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Rat Brain Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human DELE. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to 18aa from human DELE at N-terminal.
