Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions.
Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc.
between cells.
They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity.
Different tissues exhibit different Claudin composition.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 2-4ug/ml for 2 hrs at RTImmunohistochemistry (Formalin/paraffin): 1:100-1:200 for 30 min.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control:T47D cells, Skin or Breast carcinoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：125ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA, 15mM sodium azide, 40% glycerol.
Specificity：Recognizes human Claudin 1. Species Crossreactivity: monkey, mouse, canine and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Ca Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to human Claudin 1 at C-terminal. Cellular Localization: Cell membrane in skin and cytoplasmic and nuclear on breast carcinoma
