Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic- lysosomal pathway is important for normal growth control and may be defective in tumor cells (1,2).
Beclin 2 is a mammalian specific homolog of the autophagy protein Beclin 1 (3).
Like Beclin 1, Beclin 2 interacts with Bcl-2 and class III PI3K complex components.
However, Beclin 2 functions in an additional lysosomal degradation pathway and is required for ligand-induced endolysosomal degradation of several G protein-coupled receptors (3).
Beclin 2 is also required for agonist-induced lysosome-mediated degradation of EGFR in lung cancer cells, suggesting that it may also play a role in regulating other intracellular signaling pathways (4).
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 20ug/mlWestern Blot: 1-2ug/mlImmunohistochemistry (paraffin): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Jurkat Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human Beclin 2.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to 16aa from human Beclin 2 at N-terminal.
