Autophagy, the process of bulk degradation of cellular proteins through an auto- phagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells.
It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components.
This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1.
ATG14 is a subunit of a class III phosphatidylinositol (PtdIns) 3-kinase complex that targets the complex to the endoplasmic reticulum, the site of autophagosome formation.
Applications:Suitable for use in Immunofluorescence, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 20ug/mlWestern Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human Small Intestine Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human ATG14. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 18aa from human ATG14.
