The adenosine receptor ADORA2B is a member of the G protein-coupled receptor superfamily and is an integral membrane protein that stimulates adenylate cyclase activity in the presence of adenosine (1,2).
Extracellular adenosine triggers a potent anti-inflammatory response that is mediated in part by ADORA2B including the stimulation of IL-10 production (3-5).
Activation of ADORA2B can also enhance the abundance of regulatory T cells (Tregs), a class of cells that work to constrain inflammation (6).
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 20ug/ml Western Blot: 0.5-1ug/mlImmunohistochemistry (paraffin): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Mouse Colon Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human ADORA2B. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to 19aa from human ADORA2B at N-terminal.
