PHD finger protein 19 (PHF19), also known as polycomb-like protein 3 (PCL3), is a polycomb group protein that functions as an accessory subunit of the polycomb repressor complex 2 (PRC2), which represses target gene expression through methylation of histone H3 at lysine 27 by the EZH2 methyltransferase (1).
PHF19 recruits PRC2 to target genes by binding trimethylated histone H3 lysine 36, a mark of active chromatin, via its Tudor domain (2-4).
PHF19 associates with PRC2 and the histone H3 lysine 36 demethylases NO66 and FBXL10, and is required to recruit PRC2 and NO66/ FBXL10 to stem cell genes during differentiation, resulting in PRC2-mediated trimethylation of histone H3 lysine 27, loss of trimethylated histone H3 lysine 36, and transcriptional silencing (2-4).
Thus, PHF19 is critical for the proper transition of stem cell genes from the active to inactive state during differentiation of embryonic stem cells.
Applications:Suitable for use in Western Blot, Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA, 150mM sodium chloride, 50% glycerol.
Specificity：Recognizes human PHF19. Species Crossreactivity: monkey
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mk
Immunogen：Synthetic peptide corresponding to human PHF19 at N-terminal.
