Methyl-CpG-binding protein 2 (MeCP2) is the founding member of a family of methyl-CpG- binding domain (MBD) proteins that also includes MBD1, MBD2, MBD3, MBD4, MBD5 and MBD6 (1-3).
Apart from MBD3, these proteins bind methylated cytosine residues in the context of the di-nucleotide 5 ́-CG-3 ́ to establish and maintain regions of transcriptionally inactive chromatin by recruiting a variety of co-repressor proteins (2).
MeCP2 recruits histone deacetylases HDAC1 and HDAC2, and the DNA methyltransferase DNMT1 (4-6).
MBD1 couples transcriptional silencing to DNA replication and interacts with the histone methyltransferases ESET and SUV39H1 (7,8).
MBD2 and MBD3 co-purify as part of the NuRD (nucleosome remodeling and histone de-acetylation) co-repressor complex, which contains the chromatin remodeling ATPase Mi-2, HDAC1 and HDAC2 (9,10).
MBD5 and MBD6 have recently been identified and little is known regarding their protein interactions.
MBD proteins are associated with cancer and other diseases; MBD4 is best characterized for its role in DNA repair and MBD2 has been linked to intestinal cancer (11,12).
Mutations in the MeCP2 gene cause the neurologic developmental disorder Rett Syndrome (13).
MeCP2 protein levels are high in neurons, where it plays a critical role in multiple synaptic processes (14).
In response to various physiological stimuli, MeCP2 is phosphorylated on Ser421 and regulates the expression of genes controlling dendritic patterning and spine morphogenesis (14).
Disruption of this process in individuals with altered MeCP2 may cause the pathological changes seen in Rett Syndrome.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA, 150mM sodium chloride, 50% glycerol.
Specificity：Recognizes human MBD3. Species Crossreactivity: mouse, rat and monkey
Clone # USB：N87
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding Asn87 of human MBD3.
