The NF-B/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IkB proteins (1-3).
Activation occurs via phosphorylation of IkB alpha at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-kB (3-7).
IkB alpha phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines.
Kinases that phosphorylate Ikappa B at these activating sites have been identified (8).
IkB-Zeta (MAIL, INAP) is a unique IkB family member homologous to Bcl-3 and induced by IL-1 and Toll-like receptor (TLR) ligands (9-11).
Like other family members, it contains C-terminal ankyrin-repeats responsible for interaction with NF-kB, particularly p50.
Unlike classical IkB family members (a, B, E) which inhibit NF-kB translocation and are rapidly degraded upon cytokine treatment, IkB-Zeta is cytokine-inducible and localized to the nucleus where it regulates NF-kappa B DNA binding and transactivation (12-14).
Induction of IkB-Zeta is required for TLR/ IL-1 induction of a subset of NF-kB target genes, including IL-6 (15).
However, the IkB-Zeta can also inhibit transactivation of other targets, such as TNF-alpha (14,15).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 0.1mg/ml BSA, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse IkB-Zeta.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to residues surrounding Gly108 of mouse IkB-Zeta.
