84-4368-64　Rel B (Transcription factor RelB, V-rel reticuloendotheliosis viral oncogene homolog B) 100ug　350816

特徴

• RELB (v-rel reticuloendotheliosis viral oncogene homolog B) is also known as IREL.
• The International Radiation Hybrid Mapping Consortium assigned the RELB gene to chromosome 19.
• By RT-PCR and immunocytochemical analyses, Clark et al.
• (1999) showed that RELB expression correlated with dendritic cell activation.
• NF-kappa-B-inducing kinase is required for osteoclastogenesis in response to pathologic stimuli.
• Vaira et al.
• (2008) found that overexpression of Relb, but not Rela, rescued differentiation of mouse Nik -/- osteoclast precursors, indicating that blockade of the alternative NF-kappa-B pathway, rather than the classical NF-kappa-B pathway, is responsible for the osteoclastogenic defect in the absence of Nik.
• Using Relb -/- mice, they showed that Relb itself was required for Rankl-induced osteoclastogenesis in vitro and for TNF-induced bone resorption in vivo.
• Both Relb -/- and Nik -/- mice were resistant to tumor-mediated osteolysis.
• Vaira et al.
• (2008) concluded that the alternative NF-kappa-B pathway, via RELB, plays an essential and unique role in RANKL signaling toward osteoclast development.
• Applications:Suitable for use in Western Blot Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.