84-4368-63　ROC1, CT (Regulator of Cullins 1, RBX1, E3 ubiquitin-protein ligase RBX1, Ring-box 1, E3 ubiquitin protein ligase, RNF 75) 100ug　350818

特徴

• RING-box protein 1, also known as ROC1, is a protein that in humans is encoded by the RBX1 gene.
• This gene is mapped to chromosome 22q13.2 based on an alignment of the RBX1 sequence with the genomic sequence.
• ROC1 is recruited by cullin-1 to form a quaternary SCF (HOS)-ROC1 holoenzyme (with SKP1 and the BTRCP homolog HOS).
• SCF (HOS)-ROC1 binds IKK-beta-phosphorylated I-kappa-B-alpha and catalyzes its ubiquitination in the presence of ubiquitin, E1, and CDC34.
• Conclusively, ROC1 plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.