Ras-related protein Ral-A (RalA) is a protein that in humans is encoded by the RALA gene on chromosome 7.
This protein is one of two isoforms of the Ral protein, the other being RalB, and part of the Ras GTPase family.
As a Ras GTPase, RalA functions as a molecular switch that becomes active when bound to GTP and inactive when bound to GDP.
It can be activated by RalGEFs and, in turn, activate effectors in signal transduction pathways leading to biological outcomes.
Additionally, Ral proteins have been associated with the progression of several cancers, including bladder cancer and prostate cancer.
While the above functions appear to be shared between the two Ral isoforms, their differential subcellular localizations result in their differing involvement in certain biological processes.
In particular, RalA is more involved in anchorage-independent cell growth, vesicle trafficking, and cytoskeletal organization.
Moreover, RalA specifically interacts with Exo84 and Sec5 to regulate transport of membrane proteins in polarized epithelial cells and GLUT4 to the plasma membrane, as well as mitochondrial fission for cell division.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human RALA. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa165-203, DKVFFDLMREIRARKMEDSKEKNGKKKRKSLAKRI- RERC, of human RALA at C-terminal.
