Ras-related protein Rab-10 is a protein that in humans is encoded by the RAB10 gene.
RAB10 belongs to the RAS superfamily of small GTPases.
And RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking.
The RAB10 gene is mapped to chromosome 2p23.1-p22.3 by radiation hybrid analysis.
It was found that the purified recombinant GAP domain of human AS160 showed GAP activity with RAB2A, RAB8A, RAB10, and RAB14, but not with 14 other RABs.
Immunoblot analysis showed that these RABs associated with Glut4-positive vesicles in mouse adipocytes.
Thereby, it concluded that AK160 functions as a RAB GAP and that RABs may participate in GLUT4 translocation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human RB10. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa153-185, AKANINIEKAFLTLAEDILRKTPVKEPNSENVD, of human RAB10 at C-terminal.
