84-4368-52　Rab5, CT (RAB5A, Member RAS oncogene family, Ras-related protein Rab-5A) 100ug　350808

特徴

• RAB5A (Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene.
• RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway.
• The RAB5A gene is mapped on 3p24.3.
• RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling.
• It signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein (GAP).
• RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes.
• In addition, RAB5 activation is either continuous or repetitive for up to 10 minutes, but it ends before the collapse of engulfed apoptotic cells.
• Expression of a dominant-negative mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml Immunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.