84-4368-42　PGRMC1 (Progesterone Receptor Membrane Component 1, Membrane-associated progesterone receptor component 1, HPR6.6, MPR) 100ug　350783

特徴

• Progesterone receptor membrane component 1 (PGRMC1) is a protein which co-purifies with progesterone binding proteins in the liver and ovary.
• In humans, the PGRMC1 protein is encoded by the PGRMC1 gene.
• The Sigma-2 receptor was recently identified as potentially being the same as PGRMC1.
• The sole biochemical function of PGRMC1 is heme-binding.
• PGRMC1 shares key structural motifs with cytochrome b5.
• It binds and activates P450 proteins, which are important in drug, hormone and lipid metabolism.
• Also, PGRMC1 binds to PAIR-BP1 (plasminogen activator inhibitor RNA-binding protein-1).
• However, its expression outside of the reproductive tract and in males suggests multiple functions for the protein.
• These may include binding to Insig (insulin-induced gene), which regulates cholesterol synthesis.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.1-0.5ug/ml.
• Boiling paraffin sections in 10mM citrate buffer, pH 6.0, for 20min is required for staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.