84-4368-33　NUR77, CT (NR4A1, Nuclear receptor subfamily 4 group A member 1, NAK1, GFRP1, TR3, NGFIB, Early response protein NAK1, Nuclear hormone receptor NUR/77, TR3 Orphan Receptor) 100ug　350762

特徴

• NR4A1 (Nuclear receptor subfamily 4 group A member 1), also called NAK1, GFRP1, TR3, NUR77 or NGFIB, is a protein that in humans is encoded by the NR4A1 gene, which is also a member of the Nur nuclear receptor family of intracellular transcription factors.
• The NR4A1 gene is mapped on 12q13.13.
• NR4A1 is involved in cell cycle mediation, inflammation and apoptosis.
• It plays a key role in mediating inflammatory responses in macrophages.
• In addition, subcellular localization of the NR4A1 protein appears to play a key role in the survival and death of cells.
• Nr4a1 is overexpressed in Wnt1 -transformed mouse mammary cells.
• Nr4a1 is also induced by lithium, a Wnt1 mimic, and the Nr4a1 promoter is activated by lithium and beta-catenin, a Wnt1 downstream effector.
• In contrast, human NR4A1 is not upregulated by beta-catenin, indicating that this gene is regulated differently in human and mouse cells.
• Adenoviral expression of Nr4a1 induces genes involved in gluconeogenesis, stimulates glucose production both in vitro and in vivo, and raises blood glucose levels.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.