84-4368-31　MEK-7, NT (MEK7, MAP2K7, Dual specificity mitogen-activated protein kinase kinase 7, MAP kinase kinase 7, MAPKK7, JNKK2, PRKMK7, MKK7) 100ug　350735

特徴

• MAP2K7 (Mitogen-activated protein kinase kinase 7), also known as MAP kinase kinase 7, MAPKK7, JNKK2, PRKMK7 or MKK7, is an enzyme that in humans is encoded by the MAP2K7 gene.
• This protein is a member of the mitogen-activated protein kinase kinase family.
• The MKK7 protein exists as six different isoforms with three possible N-termini (a, b, and g isoforms) and two possible C-termini (1 and 2 isoforms).
• Schramek et al.
• (2011) showed that the doxorubicin-mediated DNA damage response in human A549 lung carcinoma cells caused rapid phosphorylation and upregulation of p53 (TP53).
• MKK7 knockdown reduced p53 phosphorylation, delayed p53 upregulation, and interfered with cell cycle arrest at G2/M.
• MKK7 was activated in primary lung tumors, and tumors with a p53 mutation showed even higher MKK7 phosphorylation.
• Schramek et al.
• (2011) concluded that MKK7 exerts its tumor suppressive function through p53.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml Immunohistochemistry (paraffin): 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.