84-4368-15　SOD3, NT (Extracellular superoxide dismutase [Cu-Zn], Superoxide dismutase 3, extracellular) 100ug　350837

特徴

• SOD3 (Superoxide dismutase 3), also called Superoxide dismutase, extracellular, EC-SOD, and Cu-Zn, is an enzyme that in humans is encoded by the SOD3 gene.
• This gene encodes a member of the superoxide dismutase (SOD) protein family.
• SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen.
• Hendrickson et al.
• (1990) mapped the SOD3 gene to 4pter-q21 by a study of somatic cell hybrids.
• Stern et al.
• (2003) narrowed the assignment to 4p15.3-p15.1 by somatic cell and radiation hybrid analysis, linkage mapping, and FISH.
• The product of this gene is thought to protect the brain, lungs, and other tissues from oxidative stress.
• The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix (ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen.
• A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.