84-4367-99　Oncostatin M (OSM) 100ug　350765

特徴

• OSM (Oncostatin M) is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6.
• This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines.
• It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells.
• OSM is mapped on 22q12.2.
• It has the ability to inhibit the growth of human A375 melanoma cells but not normal human fibroblasts.
• Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types.
• OSM also has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells, a function shared by LIF, CSF3, and IL6.
• The direction of gene transcription was telomeric to centromeric, with the OSM gene located upstream of the LIF gene.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.