84-4367-95　MMP8, NT (Neutrophil collagenase, Matrix metallopeptidase 8, Neutrophil collagenase) 100ug　350747

特徴

• MMP8 (Matrix metalloproteinase 8) is a member of the family of matrix metalloproteinases.
• It is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity.
• MMP8 is mapped to 11q21-q22.
• MMP8 is an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells.
• The enzyme exhibits 58% homology to human fibroblast collagenase and has the same domain structure.
• It consists of a 20-residue signal peptide, and an 80-residue propeptide that is lost on autolytic activation by cleavage of an M-L bond.
• MMP8 was found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity.
• Matrix metalloproteinases (MMPs) have fundamental roles in tumor progression, but most clinical trials with MMP inhibitors have not shown improvements in individuals with cancer.
• MMP8 has a paradoxical protective role in cancer and provides a genetic model to evaluate the molecular basis of gender differences in cancer susceptibility.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/ml Immunohistochemistry (paraffin): 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.