84-4367-51　AIM2 (Interferon-inducible protein AIM2, Absent in melanoma 2) 100ug　350541

特徴

• Interferon-inducible protein AIM2, also known as absent in melanoma 2 or simply AIM2, is a protein that in humans is encoded by the AIM2 gene.
• It is mapped to 1q22.
• AIM2 is a member of the Ifi202/IFI16 family.
• It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2.
• Though there has been virtually no biochemistry performed, a model based on cell-based or in vivo experiments has led to the current model of how AIM2 triggers the inflammasome.
• The C-terminal HIN domain binds double stranded DNA (either viral, bacterial, or even host) and acts as a cytosolic dsDNA sensor.
• This leads to the oligomerization of the inflammasome complex.
• The N-terminal pyrin domain of AIM2 interacts with the pyrin domain of another protein ASC (or Apoptosis- associated Speck-like protein containing a caspase activation and recruitment domain).
• ASC also contains a CARD domain (caspase activation and recruitment domain), that recruits procaspase-1 to the complex.
• This leads to the autoactivation of caspase-1, an enzyme that processes proinflammatory cytokines (IL-1b and IL-18).
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.