84-4367-12　Hsc 70 (Heat shock 70kD protein 8, Heat shock cognate 71kD protein, HSC70, HSC71, HSP73, HSPA10, LAP1, LPS-Associated Protein 1) 100ug　350662

特徴

• HSPA8 (heat shock 70kD protein 8) also known as HSC70, HSC71, HSP73, HSPA10, Formerly, LAP1 or LPS-Associated Protein 1, is a heat shock protein that in humans is encoded by the HSPA8 gene.
• The HSPA8 gene contains 9 exons and spans 5kb.
• The deduced HSPA8 protein has 646aa and a predicted molecular mass of 70.899kD.
• And the HSPA8 gene is mapped on 11q24.1.
• HSPA8 plays an important role in cells by transiently associating with nascent polypeptides to facilitate correct folding.
• HSP73 also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell.
• Rapid decay involves AU-rich binding protein AUF1, which complexes with heat-shock proteins HSC70 and HSP70, translation initiation factor EIF4G, and poly (A)-binding protein.
• In the absence of Il3, Hsc70 formed a complex with Hsp40 and Hip, and this complex, in association with Eif4g and Pabp, formed a high-stability complex with Bim mRNA that protected it from ribonucleases.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.