Histidine triad nucleotide-binding protein 1 is a protein that in humans is encoded by the HINT1 gene.
It is a haploinsufficient tumor suppressor gene that inhibits the Wnt/b-catenin pathway in colon cancer cells and Microphthalmia-associated transcription factor (MITF) activity in human mast cells.
The protein encoded by this gene can hydrolyze substrates such as AMP- morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2.
The encoded protein interacts with these substrates via a histidine triad motif, which is part of the loop that binds to the substrate.
Several transcript variants, but only one of them protein-coding, have been found for this gene.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human HINT1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa59-92, HISQISVAEDDDESLLGHLMIVGKKCAADLGLNK, of human HINT1.
