EIF6 (Eukaryotic Translation Initiation Factor 6), also called EIF3A or ITGB4BP, is a human gene.
By fluorescence in situ hybridization, Sanvito et al.
(1998) mapped the ITGB4BP gene to 20q11.2.
Ceci et al.
(2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6.
In the cytoplasm, EIF6 is bound to free 60S but not to 80S subunits.
Furthermore, EIF6 interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C.
Gandin et al.
(2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo.
Eif6-null mouse embryos were lethal at preimplantation.
Heterozygous mice had 50% reduction of eIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml Immunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human EIF6. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human EIF6 expressed in E. coli. Species sequence homology: mouse and rat (99.3%)
