CCL11 (Chemokine (C-C motif) ligand 11), also known as Eotaxin, is a small cytokine belonging to the CC chemokine family.
Eotaxin selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in allergic responses.
The effects of Eotaxin are mediated by its binding to a G-protein-linked receptor known as a chemokine receptor.
Chemokine receptors for which Eotaxin is a ligand include CCR2, CCR3 and CCR5.
However, it has been found that eotaxin-1 (CCL11) has high degree selectivity for its receptor, such that they are inactive on neutrophils and monocytes, which do not express CCR3.
The gene for human Eotaxin is encoded on three exons and is located on chromosome 17q12.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human Eotaxin.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Eotaxin expressed in E. coli. Species sequence homology: mouse (61.6%) and rat (63%).
