Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provides a pathway for water to exit these cells.
The gene of AQP4 is mapped to 18q11.2-q12.1.
Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127aa, 55aa, 27aa, and 92aa separated by introns of 0.8, 0.3, and 5.2 kb.
Unlike other aquaporins, an alternative coding initiation sequence (designated exon 0) was located 2.7 kb upstream of exon 1.
When spliced together, M1 and the subsequent 10aa are encoded by exon 0; the next 11aa and M23 are encoded by exon 1.
AQP4 is expressed in astrocytes and is upregulated by direct insult to the central nervous system.
AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis.
It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (frozen/paraffin): 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human Aquaporin 4. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa295-323, KPGVVHVIDVDRGEEKKGKDQSGEVLSSV, of human Aquaporin 4 at C-terminal.
