84-4366-22　VDAC1 (VDAC, Porin, Outer mitochondrial membrane protein porin 1, Voltage-dependent anion-selective channel protein 1, Voltage-dependent anion channel 1) 100ug　350852

特徴

• The voltage-dependent anion channel (VDAC) of the outer mitochondrial membrane is a small, abundant outer membrane pore-forming protein found in the outer membranes of all eukaryotic mitochondria.
• The VDAC protein is thought to form the major pathway for movement of adenine nucleotides through the outer membrane and to be the mitochondrial binding site for hexokinase and glycerol kinase.
• At low transmembrane voltage, VDAC is open for anions such as phosphate, chloride, and adenine nucleotides.
• At higher transmembrane voltage, VDAC functions as a selective channel for cations and uncharged molecules.
• These features make VDAC likely to play a role in mitochondrial energy metabolism.
• Huizing et al.
• studied by Northern and Western blot analyses the human tissue distribution of mitochondrial transmembrane metabolite carriers.
• They found that VDAC1 mRNA has a ubiquitous distribution, with most pronounced expression in heart, liver, and skeletal muscle, whereas the VDAC2 isoform appears to be expressed only in the heart.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml Immunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.