84-4365-90　GCLC (Glutamate-cysteine Ligase Catalytic Subunit, GCS Heavy Chain, Gamma-ECS Gamma-glutamylcysteine Synthetase, GLCL, GLCLC) 100ug　350635

特徴

• GCLC, also named Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene.
• Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis.
• The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit.
• The gene encoding the catalytic subunit encodes a protein of 367aa with a calculated molecular weight of 72.773kD and maps to chromosome 6p12.1.
• Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.