Glutamate decarboxylase 1 (brain, 67kD) (GAD67), also known as GAD1, is a human gene.
It is mapped to 2q31.1.
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes.
The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid.
A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes.
This gene may also play a role in the stiff man syndrome.
Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures.
Alternative splicing of this gene results in two products, the predominant 67kD form and a less-frequent 25kD form.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human GAD67. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Recombinant protein corresponding to human GAD67 expressed in E. coli. Species sequence homology: mouse, rat (95%)
