84-4365-83　Decorin (DCN) 100ug　350594

特徴

• Decorin is a protein that in humans is encoded by the DCN gene.
• This gene is mapped to 12q21.3.
• It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS).
• Decorin is a small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein.
• This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly.
• And it also may play a role in epithelial/mesenchymal interactions during organ development and shaping.
• Decorin has been shown to have anti-tumorigenic properties in an experimental murine tumor model and is capable of suppressing the growth of various tumor cell lines.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.1-0.5ug/ml.
• The detection limit is ~0.5ng/lane under reducing conditions.
• Immunohistochemistry (Paraffin): 0.5-1ug/ml.
• Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 minutess is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.