84-4365-72 IL6 (Interleukin-6) 100ug 350684
特徴
- Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene.
- IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine.
- It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation.
- IL-6 is one of the most important mediators of fever and of the acute phase response.
- IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone.
- Bowcock et al.
- (1988) assigned the IL6 gene to chromosome 7p21.
- By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, Sutherland et al.
- (1988) mapped the IL-6 gene to chromosome 7p15.
- Applications:Suitable for use in ELISA, Western Blot.
- Other applications not tested.
- Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
- Storage and Stability:Lyophilized powder may be stored at -20°C.
- Stable for 12 months after receipt at -20°C.
- Reconstitute with sterile ddH2O.
- Aliquot to avoid repeated freezing and thawing.
- Store at -20°C.
- Reconstituted product is stable for 12 months at -20°C.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Further dilutions can be made in assay buffer.
仕様
- Size:100ug
- Host:rabbit
- Source Antibody:mouse
- Grade:Affinity Purified
- Purity:Purified by immunoaffinity chromatography.
- Form:Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
- Specificity:Recognizes mouse IL6.
- Isotype:IgG
- Calc Applications Abbrev:E WB
- Calc Crossreactivity:Mo
- Immunogen:Recombinant protein corresponding to mouse IL6 expressed in E. coli. Species sequence homology: human (40%) and rat (85%)
- Shelf Life:1year
- Swiss Prot Number:P08505
- EU Commodity Code:30021010
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