Modification of proteins by the covalent attachment of ubiquitin is a key regulatory mechanism of many cellular processes including protein degradation by the 26S proteasome.
Deubiquitination, reversal of this modification, must also regulate the fate and function of ubiquitin conjugated proteins.
Deubiquitinating enzymes catalyze the removal of ubiquitin from ubiquitin conjugated substrate proteins as well as from its precursor proteins.
Deubiquitinating enzymes occupy the largest family of enzymes in the ubiquitin system, implying their diverse function in regulation of the ubiquitin mediated pathways (Kim et al 2003, PMID 12944365).
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human USP32.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa438-470 from human USP32 at N-terminal, KLH conjugated.
