May mediate the control of various cellular processes by insulin.
When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2.
Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit Ozes ON, et al.
(2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645 Tzatsos A, et al.
(2006) Mol Cell Biol; 26(1): 63-76 Kadowaki T, et al.
(2000) J Clin Invest; 106(4): 459-465 Ozes ON, et al.
(2001) Proc Natl Acad Sci U S A; 98(8): 4640-4645 Szanto I, et al.
(2000) Proc Natl Acad Sci U S A; 97(5): 2355-2360
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to the sequence around phosphorylation site of serine 636 (P-M-S(p)-P-K) derived from human IRS-1.
