Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration.
VASP promotes actin filament elongation.
It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein.
VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments.
Plays a role in actin-based mobility of Listeria monocytogenes in host cells.
Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
Zhao WM, et al.
(2001) EMBO J 20(9): 2315-2325.
Millard TH, et al.
(2005) EMBO J 24(2): 240-250.
K
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Human Mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide corresponding to the sequence around phosphorylation site of serine 157 (R-V-S(p)-N-A) derived from human VASP.
