84-4359-03　SMC1(phosphorylated Ser957) 100ul　334880

特徴

• Involved in chromosome cohesion during cell cycle and in DNA repair.
• Central component of cohesin complex.
• The cohesin complex is required for the cohesion of sister chromatids after DNA replication.
• The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped.
• At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate.
• The cohesin complex may also play a role in spindle pole assembly during mitosis.
• Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR.
• Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
• Yang XM.
• et al.
• (2004) Mol Cell Biol.
• 24(12): 5290-5303.
• Kim ST.
• et al.
• (2002) Genes Dev.
• 16(5): 560-570.
• Revenkova E.et al.
• (2001) Mol Cell Biol.
• 21(20): 6984-6998
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.