Key regulator of entry into cell division that acts as a tumor suppressor.
Acts as a transcription repressor of E2F1 target genes.
The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest.
Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation.
Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression.
Controls histone H4 'Lys-20' trimethylation.
Inhibits the intrinsic kinase activity of TAF1.
In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Roesch A, et al.
(2005) Mod Pathol.
18(4): 565-572.
Chadee DN, et al.
(2004) Nat Cell Biol.
6(8): 770-776.
Knudsen ES, et al.
(1997) Mol Cell Biol.
17(10): 5771-5783.
Knudsen ES, et al.
(1996) J Biol Chem.
271(14): 8313-8320.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to the sequence around phosphorylation site of serine 807 (Y-I-S(p)-P-L) derived from human Rb.
