ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA bindingprotein that could bind a tax-responsive enhancer element in the LTR of HTLV-1.
The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2).
The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins.
These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain.
Two alternative transcripts encoding the same protein have been described.
Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.Sato T., Shimotohno K.
J.
Virol.
65:1420-1426(1991) Leiden J.M.
Proc.
Natl.
Acad.
Sci.
U.S.A.
89:4820-4824(1992) Bridgeman A.M.
Wright H.
Nature 402:489-495(1999)
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Human Mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide corresponding to the sequence around phosphorylation site of Serine 219 (N-D-S(p)-G-T) derived from Human ATF-4.
