The function of the protein encoded by this gene is not well defined, however it contains zinc finger motifs suggestive of a transcription factor.
The protein (isoform 1) is localized to both the nucleus and cytoplasm.
Three splice variant mRNAs products have been described [1].
The longer AIRE-1 mRNA appears to be more abundant and includes exons 1 through 14.
Splice variant AIRE-2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14.
Variant AIRE-3 includes the same exon 1-8-9 sequences as found in AIRE-2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human AIRE.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to the sequence around phosphorylation site of Serine156 P-G-S(p)-Q-L) derived from human AIRE.
