84-4356-96 Ras-GRF1 (phosphorylated Ser916) 100ul 332039
特徴
- Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors).
- Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx .
- Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity.
- Multiple PKA phosphorylation sites on Ras-GRF have been identified.
- Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity.
- Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.
- Applications:Suitable for use in Immunohistochemistry.
- Other applications not tested.
- Recommended Dilution:Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
- Storage and Stability:May be stored at 4°C for short-term only.
- Aliquot to avoid repeated freezing and thawing.
- Store at -20°C.
- Aliquots are stable for 12 months after receipt.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
- Size：100ul
- Host：rabbit
- Source Antibody：human
- Grade：Affinity Purified
- Purity：Purified by immunoaffinity chromatography.
- Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
- Specificity：Mouse Rat
- Isotype：IgG
- Calc Applications Abbrev：IHC
- Calc Crossreactivity：Hu Mo Rt
- Immunogen：Peptide corresponding to the sequence around phosphorylation site of Serine 916(N-E-G(p)-T P) derived from Human Ras-GRF1.
- Shelf Life：1year
- Swiss Prot Number：Q13972
- EU Commodity Code：30021010
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