Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress.
Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'.
Activity suppressed by insulin.
Main regulator of redox balance and osteoblast numbers and controls bone mass.
Orchestrates the endocrine function of the skeleton in regulating glucose metabolism.
Acts syngernistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity.
Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP.
In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A to activate the expression of genes such as IGFBP1, G6PC and PPCK1.
Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and SKT4/MST1.
Promotes neural cell death.
Mediates insulin action on adipose.
Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake.
Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human FOXO1/O3./04 pan Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Peptide corresponding to the sequence around phosphorylation site of threonine 24/32(S-C-T(p)-W-P) derived from Human FOXO1/3/4-pan .
