Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis.
Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy.
Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly.
Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Matsuya M.et.al.
(1998)J.
Biol.
Chem.
273:1003-1014 Fujita H.et.al.
(1998)J.
Biol.
Chem.
273:26516-26521 Le Romancer M.et.al.
(2008)Mol.
Cell 31:212-221
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Human Rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Peptide corresponding to the sequence around phosphorylation site of tyrosine 397(D-D-Y(p)-A-E)derived from Human FAK.
