The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability.
Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function.
Plays a central role in DNA repair by facilitating cellular response to DNA repair.
Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle.
Involved in transcriptional regulation of P21 in response to DNA damage.
Required for FANCD2 targeting to sites of DNA damage.
May function as a transcriptional regulator.
Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.
Applications:Suitable for use in ELISA and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human BRCA1.
Isotype：IgG
Calc Applications Abbrev：E IF
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to the sequence around phosphorylation site of serine 988 (I-K-S(p)-F-V) derived from human BRCA1.
