Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development.
DNA methylation is coordinated with methylation of histones.
May preferentially methylates nucleosomal DNA within the nucleosome core region.
May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation.
Seems to be involved in gene silencing By similarity.
In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs.
Function as transcriptional corepressor by associating with ZHX1.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:500Western Blot: 1:500-1:3000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to N-terminal of human DNMT3B.
