Capable of inducing cell cycle arrest in G1 and G2 phases.
Acts as a tumor suppressor.
Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus.
This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis.
Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes.
Binds to BCL6 and down-regulates BCL6-induced transcriptional repression.
Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression.
Binds to TOP1/TOPOI and stimulates its activity.
This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation.
Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing.
Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination.
Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1.
Binds to HUWE1 and represses its ubiquitin ligase activity.
May play a role in controlling cell proliferation and apoptosis during mammary gland development.
Isoform 6 may be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform is stabilized by C1QBP.
Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:100-1:500Western Blot: 1:500-1:3000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human p14 ARF.
