84-4354-32　Ku70/80 100ul　322004

特徴

• Single-stranded DNA-dependent ATP-dependent helicase.
• Has a role in chromosome translocation.
• The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner.
• It works in the 3'-5' direction.
• Binding to DNA may be mediated by XRCC6.
• Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination.
• The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold.
• The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together.
• The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step.
• Required for osteocalcin gene expression.
• Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks.
• 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined.
• The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
• Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1:100-1:500Western Blot: 1:500-1:3000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.