84-4354-26　DNA-PK 100ul　313726

特徴

• Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage.
• Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination.
• Must be bound to DNA to express its catalytic properties.
• Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C).
• The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step.
• Required to protect and align broken ends of DNA.
• May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage.
• Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion.
• Also involved in modulation of transcription.
• Recognizes the substrate consensus sequence [ST]-Q.
• Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism.
• Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2.
• Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA.
• Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.
• Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism.
• Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation.
• Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1:100-1:500Western Blot: 1:500-1:3000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.