Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands.
Topoisomerase II makes double-strand breaks.
Indirectly involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.Ren篓 M.
Linka, Nucleic Acids Res., May 2007; 10.1093.
John R.
Jenkins, Nucleic Acids Res., Nov 1992; 20: 5587 - 5592.
Kathryn L.
Gilroy, Nucleic Acids Res., Mar 2006; 34: 1597 - 1607.
Sophie Dereuddre, Cancer Res., Oct 1997; 57: 4301 - 4308.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:3000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Human Mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to Internal of human topoisomerase II beta.
