Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, STUB1, a 22kD O-linked glycosylated isoform ofSNCAIP, SEPT5, ZNF746 and AIMP2.
Mediates monoubiquitination as well as 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context.
Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation.
Mediates 'Lys-63'-linked polyubiquitination of SNCAIP, possibly playing a role in Lewy-body formation.
Mediates monoubiquitination of BCL2, thereby acting as a positive regulator of autophagy.
Promotes the autophagic degradation of dysfunctional depolarized mitochondria (mitophagy), potentially by the ubiquitination of mitochondrial proteins.
Mediates 'Lys-48'-linked polyubiquitination of ZNF746, followed by degradation of ZNF746 by the proteasome; possibly playing a role in the regulation of neuron death.
Limits the production of reactive oxygen species (ROS).
Regulates cyclin-E during neuronal apoptosis.
In collaboration with CHPF isoform 2, may enhance cell viability and protect cells from oxidative stress.
Independently of its ubiquitin ligase activity, protects from apoptosis by the transcriptional repression of p53/TP53.
May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity.
May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis.
May represent a tumor suppressor gene.Fang Yang, J.
Biol.
Chem., Apr 2005; 280: 17154 - 17162.
Yukiko Kuroda, Hum.
Mol.
Genet., Mar 2006; 15: 883 - 895.
Iris H.
Henn, J.
Neurosci., Feb 2007; 27: 1868 - 1878.
Monica Joch, Mol.
Biol.
Cell, Jun 2007; 10.1091/mbc.E05-11-1027.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:3000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Specificity：Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human Parkin.
